UMGCC Research Program:
Hormone Responsive Cancers Program Tumor Immunology and Immunotherapy
Education/Training:
College Degree:
B.A., Goshen College
Medical Degree:
St. Louis University School of Medicine
Residency:
Internal Medicine, St. Louis University Hospitals
Fellowship:
Immunology, Immunology Branch, National Cancer Institute, NIH
Contact
Information:
Mailing Address:
Department of Pathology
22 South Greene
Baltimore MD 21201
Email:
dmann001@umaryland.edu
Phone:
410-706-7070
Fax:
410-328-2967
Research Interests:
There are 3 major research projects that are currently being pursued in Dr. Mann's laboratories. A common theme of these projects is the development of vaccines for infectious agents and cancer immunotherapy. The platform for this research is based on the biology of dendritic cells as primary antigen presenting cells and the genetic restriction of the immune response imposed by the polymorphic genes in the human major histocompatibility of locus (HLA).
In studies supported by grants from NIAID and the DOD, we are investigating the capacity of recombinant plant virus particles that have incorporated peptides from bacterial structural proteins and toxic products to generate immune responses in vitro. These studies are being conducted using human peripheral blood mononuclear cells with a diverse array of HLA Class I and Class II alleles. We will select a recombinant plant virus peptide construct that is recognized by the majority of the individuals and investigate immune responses in vivo and in non-human primates.
In studies supported by a grant from the Hasumi International Research Foundation, we are investigating the use of dendritic cells as a vehicle for the delivery of candidate vaccines for cancer immunotherapy. These studies also include the development of combinations of cytokines and chemokines that will serve as adjuvants to enhance immune responses to antigens delivered by dendritic cells.
A third project is the evaluation/characterization of the immune response in multiple myeloma patients before and after stem cell transplant. These patients have been immunized with a pneumococcal vaccine and infused with the expanded T cells at various intervals during the course of their treatment. An objective of this study is to determine optimal timing for the delivery of cancer vaccines in the context of stem cell transplantation. This study is supported by a grant from the National Cancer Institute.
Publications:
Selected from 236 peer-reviewed publications:
Mann, D.L., Rogentine, G.N. Jr., Fahey, J.L., and Nathenson, S.G., Solubilization of human leukocyte membrane isoantigens. Nature, 1968, 217, 1180-1181.
Haynes, B.F., Mann, D.L., Hemler, M.E., Schroer, J.A., Shelhamer, J.H., Eisenbarth, G.S., Strominger, J.L., Thomas, C.A., Mostowski, H.S., and Fauci, A.S., Characterization of a monoclonal antibody which defines an immunoregulatory T cell subset for immunoglobulin synthesis in man. Proceedings of the National Academy of Sciences, 1980, 77, 2914-2918
Gallo, R.C., Mann, D.L., Broder, S., Ruscetti, F.W., Maeda, M., Kalyanaraman, U.S., Robert-Guroff, M., and Reitz, M.S., Human T cell leukemic-lymphoma virus (HTLV) in T--but not B--lymphocytes from a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy Sciences USA, 1982, 79, 5680-5683
Mann, D.L., Popovic, M., Sarin, P., Murray, C., Strong, D.M., Haynes, B.F., and Gallo, R.G., Cell lines producing human T cell lymphoma virus (HTLV) have altered HLA expression. Nature, 1983, 305, 58-60
Mann, D.L., DeSantis, P., Mark, G., Pfeifer, A., Newman, M., Gibbs, N., Popovic, M., Sarngadharan, M.O., Gallo, R.C., Clark, J., and Blattner, W.A., Indirect role for retrovirus in leukemogenesis. Science, 1987, 236, 1103-1106
Mann, D.L., Gartner, S., LeSane, F., Buchow, H., and Popovic, M., HIV-1 transmission and function of virus-infected monocytes/macrophages. Journal of Immunology, 1990, 44, 2152-2158
Arthur, L.O., Bess, Jr., J.W., Sowder, II, R.C., Benveniste, R.E., Mann, D.L., Chermann, J.C., Henderson, L.E., Cellular Proteins Bound to Immunodeficiency Viruses: Implications for Pathogenesis and Vaccines. Science, 1992, 258, 1935-1938
Kaslow, R.A., Carrington, M., Apple, R., Park, L., Munoz, A., Saah, A.J., Goedert, J.J., Winkler, C., O'Brien, S.J., Rinaldo, C., Detels, R., Blattner, W., Phair, J., Erlich, H., and Mann, D.L., Influence of combinations of human major histocompatibility complex genes on the course of HIV-1 infection. Nature Medicine, 1996, 2, 405-411.
Nelson, G.W., Kaslow, R., and Mann, D.L., Frequency of HLA allele-specific peptide motifs in HIV-1 protein correlates with the alleles association with relative rates of disease progression after HIV-1 infection. Proceedings of the National Academy of Sciences, USA, 1997, 94, 9802-9807
Berlyn, K.A., Ponniah, S., Stass, S.A., Malone, J.G., Hamlin-Green, G., Lim, J.K., Cottler-Fox, M., Tricot, G., Alexander, R.B., Mann, D.L., and Malone, R.W.: Developing dendritic cell polynucleotide vaccination for prostate cancer immunotherapy. J Biotechnol, 1999, 73, 155-179
Berlyn, K.A., Schultes, B., Leveugle, B., Noujaim, A.A., Alexander, R.B., and Mann, D.L.: Generation of CD4+ and CD8+ T lymphocyte responses by dendritic cells armed with PSA/anti-PSA (antigen:antibody) complexes. Clin Immunol, 2001, 101(3), 276-83
