UMGCC Research Program:
Molecular and Structural Biology Program
Education/Training:
College Degree:
B.S., University of Wisconsin at Madison
Ph.D., University of Vermont
Post Doctoral
Degree:
University of Colorado at Boulder
Contact
Information:
Mailing Address:
Department of Pharmaceutical Sciences
Room 536
University of Maryland –School of Pharmacy
20 N. Pine St.
Baltimore, MD 21201
Email:
pshapiro@rx.umaryland.edu
Phone:
410-706-8522
Fax:
410-706-0346
Research Interests:
Our interests focus on the role of the mitogen activated protein (MAP) kinase pathways during the cell cycle. In particular, we are interested in how MAP kinases regulate mitotic events and their role in cell transformation.
We are also using computer-aided molecular modeling and biological assays to define regions on the MAP kinase proteins that determine interactions with specific substrate proteins.
Publications:
1. Raines, K.W., Cao, G-L., Lee, E.K., Rosen, G.M. and Shapiro, P.S. (2006) Neuronal nitric oxide synthase induced S-nitrosylation of H-Ras inhibits calcium ionophore-mediated extracellular signal-regulated kinase activity. Biochemical J. In Press.
2. Surabhi Dangi, Fengming Chen, and Paul Shapiro (2006) Activation of extracellular signal-regulated kinase (ERK) in G2-phase delays mitotic entry through p21CIP1. Cell Proliferation, In Press.
3. Hyukjin Cha, Surabhi Dangi, Carolyn E. Machamer and Paul Shapiro. (2006) Inhibition of mixed-lineage kinase (MLK) activity during G2-phase disrupts microtubule formation and mitotic progression in HeLa cells. Cellular Signalling, 18:93-104.
4. Chad N. Hancock, Alba Macias, Eun Kyoung Lee, Su Yeon Yu, Alexander D. MacKerell, Jr., and Paul Shapiro. (2005) Identification of novel extracellular signal-regulated kinase (ERK) docking domain inhibitors. Journal of Medicinal Chemistry, 48(14):4586-4595.
5. Surabhi Dangi, and Paul Shapiro. (2005) Cdc2-mediated inhibition of epidermal growth factor activation of the extracellular signal-regulated kinase pathway during mitosis. J. Biol. Chem., 280:24524-24531.
6. Kimberly W. Raines, Guan-Liang Cao, Supatra Porsuphatana, Pei Tsai, Gerald M. Rosen, and Paul Shapiro. (2004) Nitric Oxide Inhibition of ERK1/2 Activity in Cells Expressing Neuronal Nitric Oxide Synthase. J. Biol. Chem. 279, 3933-3940.
7. Hyukjin Cha, Chad Hancock, Surabhi Dangi, Dony Maiguel, France Carrier, and Paul Shapiro. (2004) Phosphorylation regulates nucleophosmin targeting to the centrosome during mitosis as detected by cross reactive phosphorylation specific MKK1/2 antibodies. Biochemical J. 378, 857-865.
8. Cha, H., Smith, B. L., Gallo, K., Machamer, C. E., and Shapiro, P. (2004) Phosphorylation of golgin-160 by Mixed Lineage Kinase-3 (MLK-3). Journal of Cell Science. 117:751-760.
9. Dangi, S., Cha, H., and Shapiro, P. (2003) Requirement for phosphatidylinositol-3 kinase (PI3K) activity during progression through S-phase and entry into mitosis. Cellular Signalling 15: 667-675.
10. Cha, H. and Shapiro, P. (2001) Tyrosine phosphorylated ERK associates with Golgi during G2/M phase of the cell cycle: evidence for regulation of Golgi structure. J. Cell. Biol., Vol. 153:1355-1367.
