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Researcher Information

C. David Pauza, Ph.D.
Professor

Department:
Institute of Human Virology, UMBI

UMGCC Research Program:
Tumor Immunology and Immunotherapy

Education/Training:
Post Doctoral Degree: Ph.D., University of California, Berkeley
Fellowship: Laboratory of Molecular Biology at the Medical Research Council, Cambridge, England

Contact Information:

Email: pauza@umbi.umd.edu
Phone: 410-706-1367
Fax: 410-706-5198

Research Interests:
Studies of virus transmission and pathogenesis help to define new approaches to HIV treatment and prevention. Our efforts employ laboratory, animal models, and clinical research to increase our basic understanding of HIV AIDS and to apply this knowledge for better public health. Three areas of research emphasis are: biology and biochemistry of the HIV Tat protein, the role of gamma delta T cells in viral diseases and cancer, and the development of vaccines to prevent mother to child HIV transmission during breast feeding.

The Tat protein is a required viral accessory protein. Tat affects viral replication and disease both from within and outside of cells, and may be an important addition to vaccines against HIV. We are defining the patterns and consequences of antibody binding to Tat, and designing new vaccines to improve protective immunity.

Secondly, gamma delta T cells are a minor subset in peripheral blood but are critical for microbial immunity and tumor surveillance. Our molecular studies defined the gamma delta T cell receptor repertoire and the structures required for pathogen responses. We are studying the changes in gamma delta T cells during HIV disease and exploring new therapeutic approaches for activating this subset for clinical benefit. Adoptive transfer methodologies are being used to evaluate the role for gamma delta T cells in AIDS-related and other types of human malignancies.

Lastly, my laboratory is committed to the goal of preventing mother to child transmission of HIV. Infant infection has been virtually eliminated in the developed world, but this required considerable health care resources and abstinence from breastfeeding. Alternatively, preventive vaccines may be valuable in newborns to protect against breast milk HIV transmission. Our laboratory is part of a collaborative effort to develop and implement preventive vaccines that will be integrated with conventional antiretroviral drug treatment to reduce infant infection rates.

Publications:
Tikhonov, I., Ruckwardt, T. J., Hatfield, G. S., and Pauza, C. D. (2003). Tat-neutralizing antibodies in vaccinated macaques. J Virol 77: 3157-66.

Enders, P. J., Yin, C., Martini, F., Evans, P. S., Propp, N., Poccia, F., and Pauza, C. D. (2003). HIV-Mediated gamma delta T Cell Depletion Is Specific for Vgamma2(+) Cells Expressing the Jgamma1.2 Segment. AIDS Res Hum Retroviruses 19: 21-9.

Waterman, P. M., Kitabwalla, M. M., Tikhonov, I., and Pauza, C. D. (2003). Simian/Human Immunodeficiency Virus 89.6 Expressing the Chemokine Genes MIP-1a, RANTES, or Lymphotactin. Viral Immunology 16: 35-44.

Waterman, P., Kitabwalla, M., Hatfield, G., Bryant, J., Lu, Y., Evans, P., Tikhonov, I., and Pauza, C.D. (2003). Recombinant SHIV expressing Type 1 chemokines increase survival after pathogenic virus challenge in macaques. In Press.

Horejsh, D., Ruckwardt, T., and Pauza, C. D. (2002). CXCR4-dependent HIV-1 infection of differentiated epithelial cells. Virus Research 90, 275-86.

Poccia, F., Gougeon, M. L., Agrati, C., Montesano, C., Martini, F., Pauza, C. D., Fisch, P., Wallace, M., and Malkovsky, M. (2002). Innate T-cell immunity in HIV infection: the role of Vgamma9Vdelta2 T lymphocytes. Curr Mol Med 2(8), 769-81.

Evans, P. S., Enders, P. J., Yin, C., Ruckwardt, T., Malkovsky, M., and Pauza, C. D. (2001). In vitro stimulation with a non-peptidic alkylphosphate antigen expands cells expressing the Vg2-Jg1.2/Vd2 T cell receptors. Immunology 104, 19-27.

Kuloglu, E. S., McCaslin, D. R., Kitabwalla, M., Pauza, C. D., Markley, J. L., and Volkman, B. F. (2001). Monomeric solution structure of the prototypical 'C' chemokine Lymphotactin. Biochemistry 40, 12486-96.

Pauza, C. D., Trivedi, P., Wallace, M., Ruckwardt, T. J., LeBuanec, H., Lu, W., Bizzini, B., Burny, A., Zagury, A., and Gallo, R. C. (2000). Vaccination with Tat toxoid attenuates disease in simian/human immunodeficiency virus-challenged macaques. Proc. Natl. Acad. Sci. (USA) 97, 3515-19.